RodZ regulates the post-transcriptional processing of the Shigella sonnei type III secretion system.

نویسندگان

  • Jiro Mitobe
  • Itaru Yanagihara
  • Kiyouhisa Ohnishi
  • Shouji Yamamoto
  • Makoto Ohnishi
  • Akira Ishihama
  • Haruo Watanabe
چکیده

The expression of the type III secretion system-a main determinant of virulence in Shigella-is controlled by regulator cascades VirF-InvE (VirB) and CpxAR two-component system. A screen for mutants that restore virulence in the cpxA background led to the isolation of a mutant of rodZ, a cytoskeletal protein that maintains the rod-shaped morphology of bacilli. InvE is normally repressed at 30 °C because of decreased messenger RNA (mRNA) stability, but rodZ mutants markedly increase invE-mRNA stability. Importantly, the inhibition of InvE production by RodZ can be genetically separated from its role in cell-shape maintenance, indicating that these functions are distinguishable. Thus, we propose that RodZ is a new membrane-bound RNA-binding protein that provides a scaffold for post-transcriptional regulation.

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عنوان ژورنال:
  • EMBO reports

دوره 12 9  شماره 

صفحات  -

تاریخ انتشار 2011